stepone software v2.3 (Thermo Fisher)
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stepone software v2.3
Stepone Software V2.3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stepone+software+v2%2E3/stepone+software/bio_rxiv__2025__06__05__658110-205-10-13
Average 90 stars, based on 1 article reviews
Stepone Software V2.3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stepone+software+v2%2E3/stepone+software/bio_rxiv__2025__06__05__658110-205-10-13
Average 90 stars, based on 1 article reviews
stepone software v2.3 - by Bioz Stars,
2026-09
90/100 stars
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Fluorescence:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Software:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Generated:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Recombinant:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Sequencing:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Protease Inhibitor:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Viability Assay:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Western Blot:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. SYBR Green Assay:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Membrane:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. RNA Sequencing:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Imaging:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Control:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. Real-time Polymerase Chain Reaction:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. High Content Screening:Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy. Article Snippet: Delta CT was calculated relative to the RNA28S1 housekeeping gene using Article Title: The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IL10 R 5’-ACCTTGGTCTTGGAGCTTAT-3’ This manuscript N/A Recombinant DNA pLenti-dCas9-VPR-blast Addgene Cat #96917; RRID:Addgene_96917 pLKO5.sgRNA.EFS.GFP Addgene Cat #57822; RRID:Addgene_57822 pLKO5.SAT1_1.EFS.GFP This manuscript N/A pLKO5.SAT1_2.EFS.GFP This manuscript N/A Software and Algorithms GraphPad Prism Version 10 Prism RRID:SCR_002798 FlowJo Software Version 10.8.1 FlowJo, LLC RRID:SCR_008520 FACSDiva 7 BD Biosciences RRID:SCR_001456 Cytek SpectroFlow Cytek RRID:SCR_025494 SnapGene Dotmatics RRID:SCR_015052 StepOne Software v2.3 Applied Biosystems, Thermo Fisher Scientific RRID:SCR_014281 TissueQuest v7.1 TissueGnostics RRID:SCR_014822 cellranger v6.0.1 10X Genomics https://www.10xgenomics.com/ support/software/cell-ranger/ doubletdetection v4.2 GitHub https://github.com/JonathanShor/ DoubletDetection scVI v0.15.4 PyPI https://scvi-tools.org/ scanpy v1.9.7 PyPI https://github.com/scverse/scanpy leidenalg v0.10.2 PyPI https://github.com/vtraag/leidenalg umap v0.5.3 PyPI https://github.com/lmcinnes/umap milopy v0.1.0 GitHub https://github.com/emdann/milopy rpy2 v3.5.15 PyPI https://github.com/rpy2/rpy2 R v4.0.5 (milopy) v4.3.0 (CellChat) Anaconda https://www.r-project.org/ celltypist v1.6.2 PyPI https://www.celltypist.org/ CellChat v2.1 GitHub https://github.com/jinworks/CellChat MAST v1.26.0 Bioconductor https://bioconductor.org/packages/ release/bioc/html/MAST.html Seurat v4.3.0.1 CRAN https://satijalab.org/seurat/ gseapy v1.1.1 PyPI https://github.com/zqfang/GSEApy pySCENIC v0.12 PyPI https://github.com/aertslab/pySCENIC reComBat v0.1.0 PyPI https://github.com/BorgwardtLab/reComBat arboreto v0.1.6 PyPI https://github.com/aertslab/arboreto Monocle v1.3.7 Bioconductor https://bioconductor.org/packages/ release/bioc/html/monocle.html scikit-learn v1.5.0 PyPI https://scikit-learn.org/ seaborn v0.13.2 PyPI https://seaborn.pydata.org/ Python v3.9.4 (general analysis) v3.9.2 (reComBat) v3.12.3 (pySCENIC) Anaconda https://www.python.org/ Article Title: Evaluation of a novel SYBR Green real-time PCR assay for the Klebsiella pneumoniae species complex: an accurate and fast molecular tool for public health Article Snippet: Klebsiella pneumoniae species complex (KpSC), importantly multidrug-resistant Klebsiella pneumoniae is a critical priority pathogen that causes serious infections in humans and animals.. Laboratory-developed tests were used to detect specific phylogroups, particularly the KpSC, although the translational potential has yet to be assessed due to a lack of internal validation data.. We investigated one novel SYBR Green real-time PCR method, the ZKIR (zur-khe intergenic region) assay, for identifying KpSC using human and animal KpSC (n = 53) and non-KpSC isolates (n = 39) at Vidyasagar University, West Bengal, India. Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer Article Snippet: Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye Article Snippet: Fluorescence signals were recorded during each cycle and analyzed using Article Title: Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response Article Snippet: Threshold cycles (Ct) and dissociation curves were generated automatically by StepOne and Article Title: Endoscopic liquid biopsy of bile: superior diagnostic and prognostic performance of bile-derived exosomal miR-21-5p for biliary tract cancers. Article Snippet: miR-21-5p showed superior diagnostic performance (AUC: 0.913) over serum miR-21-5p (AUC: 0.628) and CA19-9 (AUC: 0.793).. Prognostically, a higher bile miR-21-5p was associated with poorer overall survival and was identified as an independent predictor (HR: 2.446, p = 0.002), whereas serum miR-21-5p lacked significant prognostic value.. B/S ratio also showed high diagnostic accuracy when ≥ 2 (AUC: 0.870), and a B/S ratio ≥ 13 was associated with significantly poorer overall survival and found as an independent prognostic predictor (HR: 2.554, p = 0.008). Article Title: IL-7Rα signaling in regulatory T cells of adipose tissue is essential for systemic glucose homeostasis. Article Snippet: Regulatory T cells (Tregs) mediate tissue homeostasis and repair.. The function of the interleukin-7 receptor α (IL-7Rα) in nonlymphoid tissue Tregs is still unknown, although low expression of IL-7Rα is a widely accepted marker for Tregs.. Here, we show that IL-33R (ST2)–expressing Tregs in the visceral adipose tissue (VAT) express the IL-7Rα at high levels. |